Henry II, Duke of Lorraine was a brother-in-law of Henry
Family and History was the son of Charles III, Duke of Lorraine were Francis I, Duke of Lorraine. The couple were married de Saint Germain-en-Laye at the Château. No issue was a daughter of Vincenzo Gonzaga, Duke of Mantua. Margherita's sister Eleonor Gonzaga was a future Holy Roman Empress.